Proteinase K Powder 100mg
Overview
Proteinase K is a nonspecific serine protease that hydrolyzes a variety of peptide bonds. This recombinant form of proteinase K is similar to the wild type, but with increased specificity and stability at room temperature (RT). Proteinase K is active on a wide range of temperatures and buffers with optimal activity between 15 and 75°C and pH between 4 and 11. Activity is stimulated with SDS, urea and EDTA buffers.
Form
- Lyophilized White Powder.
Source
- Isolated from a recombinant yeast expressing modified gene of Tritirachium limber.
Concentration
- 34U/mg.
Size
- 100mg
Dilution Buffer
- 20 mM Tris-HCl, 1 mM CaCl , 50% glycerol or dH O, pH7,4 at 25°C. Molecular Weight: 29,3 kDa.
Storage Conditions
- Store at -20ºC.
ACTIVITY ASSAY AND UNIT DEFINITIONS
One unit will release 1 μmol of tyrosine per minute at 37º C, pH7.5. Dnase and Rna Activity: not detectable. Special quality to use in molecular biology applications as well as all electrophoresis techniques.
Application
- Isolation of DNA and RNA in a broad variety of tissues
- Glycoprotein modification in structural studies
- PCR purification; Inactivation of RNAses
- DNAses and enzymes in reaction.
Warranty Information
All products we sell are covered solely by the manufacturer's warranty. Warranty information is included with the product and is subject to the conditions set forth by the manufacturer. We include such information in the product details for each item, when ever available. The manufacturer warrants their products are free from defects in material and workmanship. Repair or replacement may be issued for any product which is found to be defective under the terms of this warranty. Wholesale Point will assist with warranty issued within the first 14 days of product purchase. After that time the product manufacturer must be contacted directly to address all warranty issues. In many cases, the manufacturer can diagnose and resolve product concerns over the phone. For more information or to obtain contact information for the manufacturer, please call us +1(786) 420-2018.
Purchasing & Delivery
We take pride in making sure your order gets out quickly. We carefully check every item before shipping and we give extra care to our packaging to make sure every item arrives safely. Orders normally ship via UPS Ground, FedEx, or USPS within 1-2 days when in stock. Some items are drop shipped from the manufacturer and can take a little longer. For your convenience, whenever we ship an order, we electronically mail a shipping confirmation notice including the tracking number from UPS, FedEx, USPS, or a local delivery service.
Drop Ship Orders
Orders that are drop shipped from the manufacturer are subject to the manufacturer's availability and shipping schedule. Some items are special order or built-to-order and can take an average of 5-7 business days to ship. We make reasonable attempts to provide and display a shipping lead time on each product we sell. If you have any questions about our shipping policy or would like to know the availability or lead time on a specific drop ship product please feel free to contact us via our Contact Us Page. If you would like a drop shipped item expedited, please place your order by phone to guarantee your order can be expedited.Payment Policy
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