Proteinase K Powder 100mg - Uniscience - Uniscience Corp.

Proteinase K Powder 100mg - Uniscience

SKU: UNI-R3142

Uniscience

Regular price $80.00 Sale

Overview

Proteinase K is a nonspecific serine protease that hydrolyzes a variety of peptide bonds. This recombinant form of proteinase K is similar to the wild type, but with increased specificity and stability at room temperature (RT). Proteinase K is active on a wide range of temperatures and buffers with optimal activity between 15 and 75°C and pH between 4 and 11. Activity is stimulated with SDS, urea and EDTA buffers.

Form

  • Lyophilized White Powder.

Source

  • Isolated from a recombinant yeast expressing modified gene of Tritirachium limber.

Concentration

  • 34U/mg.

Size

  • 100mg

Dilution Buffer

  • 20 mM Tris-HCl, 1 mM CaCl , 50% glycerol or dH O, pH7,4 at 25°C. Molecular Weight: 29,3 kDa.

Storage Conditions

  • Store at -20ºC.

ACTIVITY ASSAY AND UNIT DEFINITIONS

One unit will release 1 μmol of tyrosine per minute at 37º C, pH7.5. Dnase and Rna Activity: not detectable. Special quality to use in molecular biology applications as well as all electrophoresis techniques.

Application

  • Isolation of DNA and RNA in a broad variety of tissues
  • Glycoprotein modification in structural studies
  • PCR purification; Inactivation of RNAses
  • DNAses and enzymes in reaction. 

Download Datasheet