Reagents

LE Agarose Multi-purpose - 500g
Translation missing: en.products.product.regular_price $300.00 Sold Out
Translation missing: en.products.product.regular_price $300.00 Sold Out
Overview
Uniscience LE Agarose is a low EEO, multi-purpose, standard melting point agarose that yields high-resolution sharp DNA bands with high clarity and low background. Its optimized gel strength enhances the ease of gel processing and handling. Manufactured using innovative Organic Solvent Free Manufacturing process deal for routine DNA and RNA gel electrophoresis and blotting

Specification
- Multi-purpose
- Appearance White to off-white powder
- EEO ≤ 0.13
- Gelling Point: 36 +/- 1.5 Degrees C (1.5% gel)
- Melting Point: 88 +/- 1.5 Degrees C (1.5% gel)
- Solubility Clear colorless solution at 1g in 100ml water
- Moisture: ≤ 10%
- Gel Strength: ≥ 1200 g/cm2 (1% Gel)
- Sulfate: ≤ 0.15%
- Ash: ≤ 0.5%
- Impurities: (DNase, RNase, Protease, and Endonuclease) None Detected

Packing: 500g

LE Agarose Multi-purpose - 100g
Translation missing: en.products.product.regular_price $95.00
Translation missing: en.products.product.regular_price $95.00
Overview
Uniscience LE Agarose is a low EEO, multi-purpose, standard melting point agarose that yields high-resolution sharp DNA bands with high clarity and low background. Its optimized gel strength enhances the ease of gel processing and handling. Manufactured using innovative Organic Solvent Free Manufacturing process deal for routine DNA and RNA gel electrophoresis and blotting

Specification
- Multi-purpose
- Appearance White to off-white powder
- EEO ≤ 0.13
- Gelling Point: 36 +/- 1.5 Degrees C (1.5% gel)
- Melting Point: 88 +/- 1.5 Degrees C (1.5% gel)
- Solubility Clear colorless solution at 1g in 100ml water
- Moisture: ≤ 10%
- Gel Strength: ≥ 1200 g/cm2 (1% Gel)
- Sulfate: ≤ 0.15%
- Ash: ≤ 0.5%
- Impurities: (DNase, RNase, Protease, and Endonuclease) None Detected

Packing: 100g
UniSafe Dye Nucleic Acid Staining Solution(20,000x) 1ml
Translation missing: en.products.product.regular_price $95.00
Translation missing: en.products.product.regular_price $95.00

Overview
UniSafe Dye Nucleic Acid Staining Solution (20,000x) is a new and safe nucleic acid stain, an alternative to the traditional ethidium bromide (EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 309 nm and another at 419 nm. In addition, it has one visible excitation at 514 nm. The fluorescence emission of UniSafe Dye bound to DNA is centered at 537 nm. UniSafe Dye Nucleic Acid Staining Solution (20,000x) is as sensitive as EtBr. The staining protocol for UniSafe Dye Nucleic Acid Staining Solution (20,000x) is similar to that for EtBr. UniSafe Dye Nucleic Acid Staining Solution (20,000x) had a negative result in mouse marrow chromophilous erythrocyte micronucleus test and also in mouse spermary spermatocyte chromosomal aberration test. So it is wise to choose UniSafe Dye Nucleic acid Staining Solution (20,000x) instead of EtBr for detecting nucleic acids in agarose gels.

Characteristics
- Used for detecting double-strand DNA and single-stranded RNA
- Alternative to the ethidium bromide staining
- As sensitive as EtBr or more sensitive than that
- Non-toxic, non-mutagenic and non-carcinogenic
- No hazard waste

Content
- UniSafe Dye Nucleic Acid Staining Solution (20,000x) 1 ml

Storage Condition
- Store at room temperature and stable for 12 months.
- For more stable use, should store at 4℃ (Stable for 24 months).

Application
- Visualization of DNA and RNA bands during agarose gel electrophoresis
- Isolation of DNA fragments for subcloning without introducing mutations normally caused by EtBr.

Considerations Before Use
- UniSafe Dye Nucleic Acid Staing Solution (20,000x) is non-carcinogenic but may cause skin and eye irritations. Please wear gloves when working with the product.

Protocol
1. Prepare 100 ml of agarose gel solution (concentration from 0.8~3 %) in a 250 ml flask and mix it thoroughly. Place the flask in the microwave, heat in until the solution is completely clear (about 2~3 minutes). Note: The thickness of gel should be less than 0.5 cm since thick gels may decrease sensitivity.
2. Add 5 ㎕ of UniSafe Dye Nucleic Acid Staining Solution (20,000x) to the agarose solution. Swirl the flask gently to mix the solution and avoid forming bubbles.
3. While the agarose solution cools, pour it into the gel tray until the comb teeth are immersed about 1/4~1/2 into the agarose. Note: Repeated melting of gels containing UniSafe Dye Nucleic Acid Staining Solution (20,000x) may result in low sensitivity.
4. Allow the agarose gel to cool until solidified. Load samples on the gel and perform electrphoresis.
5. Detect the bands under UV illumination. Note: UniSafe Dye Nucleic Acid Staining Solution (20,000x) allows visualization of DNA(>50 ng) in the agarose gel under visible light. This eliminates the need for exposure to UV light, which may nick and damage DNA. The intact DNA fragments purified from agarose gel can increase the efficiency of subsequent molecular biology manipulations such as cloning, transformation, and transcription.

    For more Information Download the Datasheet

    Ultra Pure Water, DEPC Treated - 500ml
    Translation missing: en.products.product.regular_price $30.00
    Translation missing: en.products.product.regular_price $30.00
    DEPC-Treated water for PCR and other applications.

    For use in molecular biology applications, PCR reactions, 2D electrophoresis, and mass spectrometry.
     
    Molecular Grade Water™ is suitable for use in molecular biology applications which demand a high quality of water and assurance that the water is free from DNase, RNase, and protease contamination. No toxic agents, such as DEPC, are used in the manufacturing of Molecular Grade Water™, eliminating DEPC interference of enzymatic reactions.  Each lot is quality tested for the absence of RNase, DNase, and protease contamination. 


    Packing: Bottle of 500ml 

    Ultra Pure Water, Molecular Grade - 500ml
    Translation missing: en.products.product.regular_price $20.00
    Translation missing: en.products.product.regular_price $20.00
    For use in molecular biology applications, PCR reactions, 2D electrophoresis, and mass spectrometry.
     
    Molecular Grade Water™ is suitable for use in molecular biology applications which demand a high quality of water and assurance that the water is free from DNase, RNase, and protease contamination. No toxic agents, such as DEPC, are used in the manufacturing of Molecular Grade Water™, eliminating DEPC interference of enzymatic reactions.  Each lot is quality tested for the absence of RNase, DNase, and protease contamination.



    Packing: Bottle of 500ml


     

    1kb DNA Ladder 250ul
    Translation missing: en.products.product.regular_price $30.00 Sold Out
    Translation missing: en.products.product.regular_price $30.00 Sold Out

    Overview
    The 1Kbp DNA Ladder consists of 8 DNA fragments ranging from 1Kb to 10Kb (1000, 2000, 3000, 4000, 5000, 6000, 8000, 10000bp). The 4000bp band has increased intensity to serve as a reference point. The fragments may be stained with intercalating agent (e.g. UniSafe Dye®) and should be run on a 0.5-1% agarose gel for maximum visualization. It is suitable for use as molecular weight standards for agarose gel electrophoresis. 1kb DNA Ladder already contains 1x Loading dye.

    Feature
    - Ready-to-use;
    - Concentration: 90ng | μl;
    - Recommended Loading: 5μl;
    - Per lane. Range (Kb): 1–10;
    - Number of Bands: 8.

    Storage Buffer
    - 10mM Tris-HCl (pH 8.0), 1mM EDTA.

    Storage Temperature
    - Store at –20°C.

    Size
    - 250μl

    Figure

    0.8% agarose gel in 1x TAE buffer

    Download Datasheet

    Proteinase K Powder 100mg
    Translation missing: en.products.product.regular_price $86.00 Sold Out
    Translation missing: en.products.product.regular_price $86.00 Sold Out

    Overview

    Proteinase K is a nonspecific serine protease that hydrolyzes a variety of peptide bonds. This recombinant form of proteinase K is similar to the wild type, but with increased specificity and stability at room temperature (RT). Proteinase K is active on a wide range of temperatures and buffers with optimal activity between 15 and 75°C and pH between 4 and 11. Activity is stimulated with SDS, urea and EDTA buffers.

    Form

    • Lyophilized White Powder.

    Source

    • Isolated from a recombinant yeast expressing modified gene of Tritirachium limber.

    Concentration

    • 34U/mg.

    Size

    • 100mg

    Dilution Buffer

    • 20 mM Tris-HCl, 1 mM CaCl , 50% glycerol or dH O, pH7,4 at 25°C. Molecular Weight: 29,3 kDa.

    Storage Conditions

    • Store at -20ºC.

    ACTIVITY ASSAY AND UNIT DEFINITIONS

    One unit will release 1 μmol of tyrosine per minute at 37º C, pH7.5. Dnase and Rna Activity: not detectable. Special quality to use in molecular biology applications as well as all electrophoresis techniques.

    Application

    • Isolation of DNA and RNA in a broad variety of tissues
    • Glycoprotein modification in structural studies
    • PCR purification; Inactivation of RNAses
    • DNAses and enzymes in reaction. 

    Download Datasheet

    1Kbp Plus DNA ladder Kit Size: 250ul / 500ul
    Translation missing: en.products.product.regular_price $30.00 Sold Out
    Translation missing: en.products.product.regular_price $30.00 Sold Out

    Overview
    The 1Kb plus DNA Ladder consists of 12 DNA fragments ranging from 1Kbp to 10Kbp (300, 500, 800, 1000, 1500, 2000, 3000, 4000, 5000, 6000, 8000, 10000bp). The 3000bp band has increased intensity to serve as a reference point. The fragments may be stained with intercalating agent (e.g. UniSafe Dye®) should be run on a 0.5-1% agarose gel for maximum visualization. It is suitable for use as molecular weight standards for agarose gel electrophoresis. The 1KB plus DNA Ladder already contains 1x Loading dye.

    Feature
    - Ready-to-use;
    - Concentration: 100ng | μl;
    - Recommended Loading: 5μl; Per lane;
    - Range (Kbp): 0.3–10.
    - Number of Bands: 12.

    Storage Buffer
    - 10mM Tris-HCl (pH 8.0), 1mM EDTA.

    Storage Temperature
    - Store at –20°C.

    Size Kit Size
    - 250μl - 500μl

    Figure

    0.8% agarose gel in 1x TAE buffer

    Download Datasheet

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