Taq DNA Polymerase is the most common PCR enzyme for amplifying up to 10kb λDNA templates and up to 3kb genomic templates. However, it is not able to amplify much longer DNA fragments. To overcome this constraint, PCR systems with a higher efficiency have been developed, such as Uni-Taq DNA Polymerase High Fidelity. This High Fidelity Polymerase is developed for amplifying long length fragments with up to 20kb from human genomic DNA, and up to 30kb from a DNA template, as well as complex sequences, without excluding short lenght fragments and simple sequences, being the most versatile enzyme for a PCR routine.
- Store at -20ºC. If stored properly, up to one year stability.
- Increased fidelity of PCR amplification, once Uni-Taq DNA Polymerase High Fidelity enzyme blend combines the proofreading activity of Pfu DNA Polymerase with the high processivity of Taq DNA Polymerase.
- Increased yield of PCR amplification, once the accuracy of the enzyme blend reduces the number of truncated amplification products formed.
- Improved performance of long PCR, once the reaction buffer and the enzyme blend are optimized for generation of certain length products.
- Standard and long PCR;
- PCR with complex templates;
- Cloning with TA and blunt ends.
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Purchasing & Delivery
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