The polymerase chain reaction (PCR) is an enzyme-mediated gene amplification technique. PCR is now a common tool used in medical and biological research labs for a variety of tasks, such as sequencing of genes, cloning of genes, hereditary diseases diagnosis, genetic fingerprints identification (used in forensics and paternity testing), infectious diseases detection and diagnosis, and transgenic organisms creation. In the beginning, the enzyme used in PCR was E. coli DNA polymerase, and this enzyme had to be added at every step of the process due to its thermal instability. Since Taq DNA polymerase was developed from Thermus aquaticus bacteria which thrives in hot spa, the contemporary automatic PCR has been available. Taq DNA polymerase optimally synthesizes DNA at 72ºC, therefore it could stably amplify a specified oligo sequence without adding enzyme at every step due to its thermal stability even at 94ºC. Uni-Taq DNA Polymerase is suitable for standard PCR and special PCR applications. Specially designed reaction buffer provides robust performance for reproducible results in several types of PCR applications as well as high efficiency and high specificity.
Store at -20°C.
High efficiency and high specificity of amplification due to specially designed PCR buffer.
Applicable to general PCR, RT-PCR, differential display, multiplex PCR, and PCR-based DNA fingerprinting (VNTR, STR, and RAPD).