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2x UNI-SYBR qPCR master mix (no ROX)2x SYBR qPCR mix - 1.25ml / ROX reference dye (50 μmol) - 25μl

2x UNI-SYBR qPCR master mix (no ROX)2x SYBR qPCR mix - 1.25ml / ROX reference dye (50 μmol) - 25μl - Uniscience Corp.
$70.00 (UNI-R20611)

Overview

2x UNI-SYBR qPCR master mix (no ROX) is a convenient premixed 2x concentrated solution which includes: Hot Star Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dye, Mg + and ROX reference dye (separate tube). The mixture can be applied for target detection of genomic DNA and cDNA. The SYBR Green I dye binds to dsDNA without using sequence-specific probes. The modified Hot Star Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of primers and template. The unique PCR buffer components and the hot start enzyme ensure PCR specificity and sensitivity. ROX is a dye molecule that can be used to normalize the well-to-well fluorescent variation and can be applied to all qPCR instruments that uses ROX as a reference dye.

Shipping and Storage

  • Store in dark at -20°C for up to 1 year.
  • For frequent use, store at +2 to +8°C for short-term (1 week), and avoiding freeze/thawing cycles.

Application

  • Gene expression analysis;
  • Gene copy number analysis.

Feature

  • High sensitivity: exactly quantify the low copy template;
  • High specificity: reduction of primer dimers and non-specific products;
  • Wide linear range: accurate quantification.

Protocol

The following example should be optimized according to the template, primer structure and target fragment size.

 1. Set the reaction system according to the following table.

Note:

1) The concentration of the primer should vary between 0.1-1.0 μM, being 0.2 μM the optimized amount. Increase the concentration of primer when amplification is not high. Reduce the concentration when non-specific reaction appears.

2) The recommended DNA template amount is between 10-100 ng of genomic DNA or 1-10 ng cDNA. As the target gene copy numbers are different among species, the template can be gradually diluted to get an optimal template amount.

Download Datasheet

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Overview

2x UNI-SYBR qPCR master mix (no ROX) is a convenient premixed 2x concentrated solution which includes: Hot Star Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dye, Mg + and ROX reference dye (separate tube). The mixture can be applied for target detection of genomic DNA and cDNA. The SYBR Green I dye binds to dsDNA without using sequence-specific probes. The modified Hot Star Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of primers and template. The unique PCR buffer components and the hot start enzyme ensure PCR specificity and sensitivity. ROX is a dye molecule that can be used to normalize the well-to-well fluorescent variation and can be applied to all qPCR instruments that uses ROX as a reference dye.

Shipping and Storage

  • Store in dark at -20°C for up to 1 year.
  • For frequent use, store at +2 to +8°C for short-term (1 week), and avoiding freeze/thawing cycles.

Application

  • Gene expression analysis;
  • Gene copy number analysis.

Feature

  • High sensitivity: exactly quantify the low copy template;
  • High specificity: reduction of primer dimers and non-specific products;
  • Wide linear range: accurate quantification.

Protocol

The following example should be optimized according to the template, primer structure and target fragment size.

 1. Set the reaction system according to the following table.

Note:

1) The concentration of the primer should vary between 0.1-1.0 μM, being 0.2 μM the optimized amount. Increase the concentration of primer when amplification is not high. Reduce the concentration when non-specific reaction appears.

2) The recommended DNA template amount is between 10-100 ng of genomic DNA or 1-10 ng cDNA. As the target gene copy numbers are different among species, the template can be gradually diluted to get an optimal template amount.

Download Datasheet

Warranty Information

All products we sell are covered solely by the manufacturer's warranty. Warranty information is included with the product and is subject to the conditions set forth by the manufacturer. We include such information in the product details for each item, when ever available. The manufacturer warrants their products are free from defects in material and workmanship. Repair or replacement may be issued for any product which is found to be defective under the terms of this warranty. Wholesale Point will assist with warranty issued within the first 14 days of product purchase. After that time the product manufacturer must be contacted directly to address all warranty issues. In many cases, the manufacturer can diagnose and resolve product concerns over the phone. For more information or to obtain contact information for the manufacturer, please call us +1(786) 420-2018.

Purchasing & Delivery

We take pride in making sure your order gets out quickly. We carefully check every item before shipping and we give extra care to our packaging to make sure every item arrives safely. Orders normally ship via UPS Ground, FedEx, or USPS within 1-2 days when in stock. Some items are drop shipped from the manufacturer and can take a little longer. For your convenience, whenever we ship an order, we electronically mail a shipping confirmation notice including the tracking number from UPS, FedEx, USPS, or a local delivery service.

Drop Ship Orders

Orders that are drop shipped from the manufacturer are subject to the manufacturer's availability and shipping schedule. Some items are special order or built-to-order and can take an average of 5-7 business days to ship. We make reasonable attempts to provide and display a shipping lead time on each product we sell. If you have any questions about our shipping policy or would like to know the availability or lead time on a specific drop ship product please feel free to contact us via our Contact Us Page. If you would like a drop shipped item expedited, please place your order by phone to guarantee your order can be expedited.

Payment Policy

We accept Visa, MasterCard, PayPal, American Express, and Discover.

Please note: When the checkout/customer details section asks for your "personal information", please enter your full name and billing address. The name and address you enter must match the information you have on file with your credit card provider, or the order will not be authorized.  If your credit card gets declined for any reason, a charge may show pending on your account.  This charge will fall off usually within 24-48 hours depending on your bank.

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