Molecular Biology Specifications
IPTG is an extremely powerful inducer, which is not metabolized by bacteria and is very stable. Therefore, it is widely used in the laboratory. IPTG is used in cloning experiments that require the induction of beta-galactosidase activity. It is often used in conjunction with the x-gal or bluo-gal to reorganize the blue-white screening of bacterial colonies that can induce the expression of lac manipulators in e. coli. IPTG plays a role in binding and altering the conformation of lacI repressors to prevent the inhibition of the beta-galactosidase encoding gene lacZ.
- +2 to +8 ºC
Avoid contact with skin and eyes. Avoid formation of dust and
aerosols.Provide appropriate exhaust ventilation at places where dust is
formed.See Material Safety Data Sheet for additional information.
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