Reagents
Uniscience Corp Biology Reagents
Uniscience Corp provides Core Bioreagents, Molecular Reagents and Bioreagents that are Pre-qualified, High-Purity, High-quality reagents that meet your life science needs, all from one source. We offer DNA Ladder & DNA Ladder Kits, UniSafe Dye Nucleic Acid Staining Solution, Ultra Pure Water, EtBr and SYBR Dye Decontamination plus much more,
Overview
2x UNI SYBR qPCR master mix (low ROX) is a convenient premixed 2x concentrated solution that includes: Hot Star Taq DNA polymerase, PCR buffer, dNTPs, SYBR Green I fluorescent dye, Mg +, and ROX reference dye. The mixture can be applied for the target detection of genomic DNA and cDNA. The SYBR Green I dye binds to dsDNA without using sequence specific probes. The chemically modified Hot Star Taq DNA polymerase is inactive at room temperature which effectively avoids the non-specific amplification caused by primer dimers or the nonspecific binding of primers and templates. The unique PCR buffer components and the hot start enzyme ensure PCR specificity and sensitivity. ROX is a dye molecule that can be used to normalize the well-to-well fluorescent variation. Suitable for ABI Prism 7500/7500 Fast, Stratagene Mx3000/Mx3005P. Corbett Rotor-Gene 3000 and other low rox level PCR instruments. This kit contains a low ROX concentration.
Shipping and Storage
- Store in the dark at -20°C for up to 1 year.
- For frequent use, store at +2 to +8°C for short-term (1 week), and avoiding freeze/thawing cycles.
Application
- Gene expression analysis;
- Gene copy number analysis.
Feature
- High sensitivity: exactly quantify the low copy template;
- High specificity: reduction of primer-dimers and non-specific products;
- Wide linear range: accurate quantification.
Protocol
- The following example should be optimized according to the template, primer structure, and target fragment size.
1. Set the reaction system according to the following table.
Note:
1) The concentration of the primer should vary between 0.1-1.0 μM, being 0.2 μM the optimized amount. Increase the concentration of primer when amplification is not high. Reduce the concentration when a non-specific reaction appears.
2) The recommended DNA template amount is between 10-100 ng of genomic DNA or 1-10 ng cDNA. As the target gene copy numbers are different among species, the template can be gradually diluted to get an optimal template amount. 2. PCR reaction conditions:
Overview
dNTP Mix is an premixed solution in pH 7.0 containing dATP, dCTP, dGTP and dTTP, each one at a final concentration of 10 mM. dNTP mix is a readyto-use solution designed to save time and to provide higher reproducibility in PCR reaction and other applications.
Storage Temperature
- Long Term:-70ºC
- Daily/Weekly use: -20ºC
Quality Control
- Functionally tested in PCR with Taq and Pfu DNA Polymerases.
- Greater than 99% purity of each component confirmed by HPLC.
- Free of endodeoxyribonuclease, exodeoxyribonuclease, ribonuclease and phosphatase activities. Applications For use in all molecular biology applications, including:
- PCR,
- long PCR,
- real-time PCR,
- high fidelity PCR,
- RT-PCR,
- cDNA synthesis,
- primer extension,
- and DNA labeling.
Overview
Molecular Biology Specifications
Recommended Use
The buffer range of MOPS is between 6.5 ~ 7.9, which is suitable for electron transfer and phosphorylation study of a thin layer of the chloroplast. It can be prepared into a variety of AGAR mediums and used as a non-toxic buffer in the limit medium to be used in the cultivation of the rod Streptomyces and the production of cephalosporin. It can be used as the electrolyte system component of medium electrophoresis (IEF) in twodimensional
gel electrophoresis (IEF). It can also be used in Northern hybridization as a buffer for the separation and trans-membrane of RNA.
Storage: Room Temperature
Size: 100g
Warning: See Material Safety Data Sheet for additional information.
Overview
Molecular Biology Specifications
Recommended Use
The buffer range of MOPS is between 6.5 ~ 7.9, which is suitable for electron transfer and phosphorylation study of a thin layer of the chloroplast. It can be prepared into a variety of AGAR medium and used as a non-toxic buffer in the limit medium to be used in the cultivation of the rod Streptomyces and the production of cephalosporin. It can be used as the electrolyte system component of medium electrophoresis (IEF) in twodimensional
gel electrophoresis (IEF). It can also be used in Northern hybridization as a buffer for the separation and trans-membrane of RNA.
Storage: Room Temperature
Size: 250g
Warning: See Material Safety Data Sheet for additional information.
Overview
Proteinase K is a nonspecific serine protease that hydrolyzes a variety of peptide bonds. This recombinant form of proteinase K is similar to the wild type, but with increased specificity and stability at room temperature (RT). Proteinase K is active on a wide range of temperatures and buffers with optimal activity between 15 and 75°C and pH between 4 and 11. Activity is stimulated with SDS, urea and EDTA buffers.
Form
- Lyophilized White Powder.
Source
- Isolated from a recombinant yeast expressing modified gene of Tritirachium limber.
Concentration
- 34U/mg.
Size
- 100mg
Dilution Buffer
- 20 mM Tris-HCl, 1 mM CaCl , 50% glycerol or dH O, pH7,4 at 25°C. Molecular Weight: 29,3 kDa.
Storage Conditions
- Store at -20ºC.
ACTIVITY ASSAY AND UNIT DEFINITIONS
One unit will release 1 μmol of tyrosine per minute at 37º C, pH7.5. Dnase and Rna Activity: not detectable. Special quality to use in molecular biology applications as well as all electrophoresis techniques.
Application
- Isolation of DNA and RNA in a broad variety of tissues
- Glycoprotein modification in structural studies
- PCR purification; Inactivation of RNAses
- DNAses and enzymes in reaction.
U-TRINZOL reagent, when stored in indicated temperature is stable for 12 months. Reagents required, but not supplied: 1. Chloroform 2. Isopropyl alcohol 3. 75% Ethanol (in RNA-free water) 4. RNase-free water or 0.5% SDS solution WARNING: Toxic in contact with skin and if swallowed causes burns. After contact with skin, wash immediately with plenty of detergent and water. If you feel unwell, seek medical advice (show label when possible). Phenol (108-95-2) and other Components (NJTSRN 80100437-5000p). PRECAUTIONS FOR PREVENTING RNASE CONTAMINATION The following guidelines should be conducted when working with RNA. 1. Always wear disposable gloves. 2. Use sterile, disposable plasticware and automatic pipettes reserved for RNA work to prevent cross-contamination with RNases from shared equipment. 3. In the presence of U-TRINZOL Reagent, RNA is protected from RNase contamination. Downstream sample handling requires nondisposable glassware or plasticware to be RNase-free. Glass items can be baked at 150°C for 4 hours and plastic items can be soaked for 10 minutes in 0.5 M NaOH, rinsed thoroughly with water and autoclaved. RECOMMENDED U-TRINZOL VOLUME ON DIFFERENT STARTING MATERIALS 
1. HOMOGENIZATION Use U-TRINZOL Reagent to prepare lysates from various sample types as described below. 1 a. TISSUES: From animal or plant (either fresh or frozen at -70°C until use) can be processed by freezing with liquid nitrogen and grinding into a fine powder using a mortar and pestle. Homogenize tissue samples in 1 ml U-TRINZOL Reagent per 50–100 mg tissue using a tissue homogenizer or rotor-stator. 1b. ADHERENT CELLS: Lyse cells directly in a culture dish by adding 1 ml of U-TRINZOL Reagent to a 3.5 cm diameter dish and passing the cell lysate several times through a pipette. The amount of U-TRINZOL Reagent added is based on the area of the culture dish (1 ml per 10 cm ) and not on the number of cells present. An insufficient amount of U-TRINZOL Reagent may result in contamination of the isolated RNA with DNA. 1c. SUSPENSION CELLS: Pellet cells by centrifugation. Lyse cells in U-TRINZOL Reagent by repetitive pipetting. Use 1 ml of the reagent per 5-10 × 10 of animal, plant or yeast cells, or per 1 × 10 bacterial cells. Washing cells before addition of U-TRINZOL Reagent should be avoided as this increases the possibility of mRNA degradation. Disruption of some yeast and bacterial cells may require the use of a homogenizer. OPTIONAL: An additional isolation step may be required for samples with high content of proteins, fat, polysaccharides or extracellular material such as: muscles, fat tissue, and tuberous parts of plants. Following homogenization, remove insoluble material from the homogenate by centrifugation at 12,000 × g for 10 minutes at 2 to 8°C. The resulting pellet contains extracellular membranes, polysaccharides, and high molecular weight DNA, while the supernatant contains RNA. In samples from fat tissue, an excess of fat collects as a top layer which should be removed. In each case, transfer the cleared homogenate solution to a fresh tube and proceed with chloroform addition and phase separation as described. 2. PHASE SEPARATION Incubate the homogenized samples for 5 minutes at 15 to 30°C to permit the complete dissociation of nucleoprotein complexes. Add 0.2 ml of chloroform per 1 ml of U-TRINZOL Reagent. Cap sample tubes securely. Shake tubes vigorously by hand for 15 seconds and incubate them at room temperature for 2 to 3 minutes. Centrifuge the samples at 12,000 × g for 5-10 minutes at 4°C. Following centrifugation, the mixture separates into a lower yellow, phenol-chloroform phase, an interphase, and a colorless upper aqueous phase. RNA remains exclusively in the aqueous phase. The volume of the aqueous phase is about 60% of the volume of U-TRINZOL Reagent used for homogenization. 3. RNA PRECIPITATION Transfer the aqueous phase to a fresh tube, and save the organic phase if isolation of DNA or protein is desired. Precipitate the RNA from the aqueous phase by mixing with isopropyl alcohol. Use 0.5 ml of isopropyl alcohol per 1 ml of U-TRINZOL Reagent used for the initial homogenization. Mix well by inverting the tube several times. Incubate samples at 15 to 30°C for 10 minutes and centrifuge at 12,000 × g for 5-10 minutes at 4°C. The RNA precipitate, often invisible before centrifugation, forms a gel-like pellet on the side and bottom of the tube.
U-TRINZOL reagent, when stored in indicated temperature is stable for 12 months. Reagents required, but not supplied: 1. Chloroform 2. Isopropyl alcohol 3. 75% Ethanol (in RNA-free water) 4. RNase-free water or 0.5% SDS solution WARNING: Toxic in contact with skin and if swallowed causes burns. After contact with skin, wash immediately with plenty of detergent and water. If you feel unwell, seek medical advice (show label when possible). Phenol (108-95-2) and other Components (NJTSRN 80100437-5000p). PRECAUTIONS FOR PREVENTING RNASE CONTAMINATION The following guidelines should be conducted when working with RNA. 1. Always wear disposable gloves. 2. Use sterile, disposable plasticware and automatic pipettes reserved for RNA work to prevent cross-contamination with RNases from shared equipment. 3. In the presence of U-TRINZOL Reagent, RNA is protected from RNase contamination. Downstream sample handling requires nondisposable glassware or plasticware to be RNase-free. Glass items can be baked at 150°C for 4 hours and plastic items can be soaked for 10 minutes in 0.5 M NaOH, rinsed thoroughly with water and autoclaved. RECOMMENDED U-TRINZOL VOLUME ON DIFFERENT STARTING MATERIALS 1. HOMOGENIZATION Use U-TRINZOL Reagent to prepare lysates from various sample types as described below. 1 a. TISSUES: From animal or plant (either fresh or frozen at -70°C until use) can be processed by freezing with liquid nitrogen and grinding into a fine powder using a mortar and pestle. Homogenize tissue samples in 1 ml U-TRINZOL Reagent per 50–100 mg tissue using a tissue homogenizer or rotor-stator. 1b. ADHERENT CELLS: Lyse cells directly in a culture dish by adding 1 ml of U-TRINZOL Reagent to a 3.5 cm diameter dish and passing the cell lysate several times through a pipette. The amount of U-TRINZOL Reagent added is based on the area of the culture dish (1 ml per 10 cm ) and not on the number of cells present. An insufficient amount of U-TRINZOL Reagent may result in contamination of the isolated RNA with DNA. 1c. SUSPENSION CELLS: Pellet cells by centrifugation. Lyse cells in U-TRINZOL Reagent by repetitive pipetting. Use 1 ml of the reagent per 5-10 × 10 of animal, plant or yeast cells, or per 1 × 10 bacterial cells. Washing cells before addition of U-TRINZOL Reagent should be avoided as this increases the possibility of mRNA degradation. Disruption of some yeast and bacterial cells may require the use of a homogenizer. OPTIONAL: An additional isolation step may be required for samples with high content of proteins, fat, polysaccharides or extracellular material such as: muscles, fat tissue, and tuberous parts of plants. Following homogenization, remove insoluble material from the homogenate by centrifugation at 12,000 × g for 10 minutes at 2 to 8°C. The resulting pellet contains extracellular membranes, polysaccharides, and high molecular weight DNA, while the supernatant contains RNA. In samples from fat tissue, an excess of fat collects as a top layer which should be removed. In each case, transfer the cleared homogenate solution to a fresh tube and proceed with chloroform addition and phase separation as described. 2. PHASE SEPARATION Incubate the homogenized samples for 5 minutes at 15 to 30°C to permit the complete dissociation of nucleoprotein complexes. Add 0.2 ml of chloroform per 1 ml of U-TRINZOL Reagent. Cap sample tubes securely. Shake tubes vigorously by hand for 15 seconds and incubate them at room temperature for 2 to 3 minutes. Centrifuge the samples at 12,000 × g for 5-10 minutes at 4°C. Following centrifugation, the mixture separates into a lower yellow, phenol-chloroform phase, an interphase, and a colorless upper aqueous phase. RNA remains exclusively in the aqueous phase. The volume of the aqueous phase is about 60% of the volume of U-TRINZOL Reagent used for homogenization. 3. RNA PRECIPITATION Transfer the aqueous phase to a fresh tube, and save the organic phase if isolation of DNA or protein is desired. Precipitate the RNA from the aqueous phase by mixing with isopropyl alcohol. Use 0.5 ml of isopropyl alcohol per 1 ml of U-TRINZOL Reagent used for the initial homogenization. Mix well by inverting the tube several times. Incubate samples at 15 to 30°C for 10 minutes and centrifuge at 12,000 × g for 5-10 minutes at 4°C. The RNA precipitate, often invisible before centrifugation, forms a gel-like pellet on the side and bottom of the tube.
Packing: Bottle of 500ml
Packing: Bottle of 500ml
Overview
Uni-Strip PCR Master Mix is a innovative and original product which is adapted to manufacture PCR Premix. Uni-Strip PCR Master Mix technology guarantees effective activity and stability for up to six months at room temperature. Uni-Strip PCR Master Mix has all components for PCR amplification dried in the PCR tubes.
STORAGE
- Shipping at room temperature.
- Storage condition: Store the product at -22 ~ -18ºC after receiving.
- Expiration: Uni-Strip PCR Master Mix can be stored for up to 24 months at -20°C and up to 6 months at RT.
CHARACTERISTICS
- Simple and fast to handle;
- High sensitivity, yield and reproducibility;
- Minimized variation by user;
- Ready and easy to use;
- Improved stability.
APPLICATIONS
- Genomic DNA PCR;
- RT-PCR (2 nd round PCR of RT-PCR);
- Direct sequencing related PCR;
- T/A vector cloning
ADDITIONAL REQUIRED ITENS
- Distilled water;
- Pipets and pipet tips (filtered);
- Mineral oil (only if the thermal cycler does not have a heated lid).
- Primers;
- Thermal cycler;
Overview
The polymerase chain reaction (PCR) is an enzyme-mediated gene amplification technique. PCR is now a common tool used in medical and biological research labs for a variety of tasks, such as sequencing of genes, cloning of genes, hereditary diseases diagnosis, genetic fingerprints identification (used in forensics and paternity testing), infectious diseases detection and diagnosis, and transgenic organisms creation. In the beginning, the enzyme used in PCR was E. coli DNA polymerase, and this enzyme had to be added at every step of the process due to its thermal instability. Since Taq DNA polymerase was developed from Thermus aquaticus bacteria which thrives in hot spa, the contemporary automatic PCR has been available. Taq DNA polymerase optimally synthesizes DNA at 72ºC, therefore it could stably amplify a specified oligo sequence without adding enzyme at every step due to its thermal stability even at 94ºC. Uni-Taq DNA Polymerase is suitable for standard PCR and special PCR applications. Specially designed reaction buffer provides robust performance for reproducible results in several types of PCR applications as well as high efficiency and high specificity.
Storage
- Store at -20°C.
Characteristics
- High efficiency and high specificity of amplification due to specially designed PCR buffer.
- Applicable to general PCR, RT-PCR, differential display, multiplex PCR, and PCR-based DNA fingerprinting (VNTR, STR, and RAPD).
Applications
- Genomic DNA PCR;
- RT-PCR;
- Direct sequencing related PCR;
- T/A vector cloning;
- LOH or MSI analysis related PCR.
Overview
Taq DNA Polymerase is the most common PCR enzyme for amplifying up to 10kb λDNA templates and up to 3kb genomic templates. However, it is not able to amplify much longer DNA fragments. To overcome this constraint, PCR systems with a higher efficiency have been developed, such as Uni-Taq DNA Polymerase High Fidelity. This High Fidelity Polymerase is developed for amplifying long length fragments with up to 20kb from human genomic DNA, and up to 30kb from a DNA template, as well as complex sequences, without excluding short lenght fragments and simple sequences, being the most versatile enzyme for a PCR routine.
Storage
- Store at -20ºC. If stored properly, up to one year stability.
Characteristics
- Increased fidelity of PCR amplification, once Uni-Taq DNA Polymerase High Fidelity enzyme blend combines the proofreading activity of Pfu DNA Polymerase with the high processivity of Taq DNA Polymerase.
- Increased yield of PCR amplification, once the accuracy of the enzyme blend reduces the number of truncated amplification products formed.
- Improved performance of long PCR, once the reaction buffer and the enzyme blend are optimized for generation of certain length products.
Applications
- Standard and long PCR;
- PCR with complex templates;
- Cloning with TA and blunt ends.